Ir. T. Ruttink : ENOD40 affects phytohormone cross-talk

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5 Dec 2003 16:00
Unit: Wageningen UR
Location: Aula (gebouw 362), Gen. Foulkesweg 1, Wageningen
Promotor: prof.dr. A.H.J. Bisseling (Molecular Biology - Development Biology of Plants)
Co Promotor: dr. H.G.J.M. Franssen

 In this thesis we developed bio-assays to study the function of ENOD40 in relation to phytohormones. By quantifying the parameters cell-elongation growth and cell-division frequency under various phytohormone regimes in transgenic tobacco BY-2 cell suspensions, we showed that the relative rates of both cellulair processes become coupled by overexpression of ENOD40. Since elongation growth is regulated by ethylene and cell division by the cytokinin/auxin ratio, our data indicate that ENOD40 is involved in cross-talk between ethylene, auxin and cytokinin. We present an overview of ENOD40 expression patterns and the structure of ENOD40 transcripts. These are characterized by two short conserved nucleotide sequence regions, which both contain biological activity in the bio-assay, and one of the two regions encodes a remarkably short oligopeptide. Only a small number of phytohormones are sufficient to regulate virtually every aspect of plant growth and development. Hence, adequate communication between phytohormones is important to coordinate cellular processes during development. Our data support understanding of how so few phytohormones can regulate such a diversity of processes, and which other components are involved in the regulatory mechanisms.
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