Dhr. M.N. (Nadeem) Khan: “A molecular cytogenetic study of intergenomic recombination and introgression of chromosomal segments in lilies (Lilium)”

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3 Jun 2009 13:30
Unit: Wageningen University
Location: Aula, building 362, Gen. Foulkesweg 1, Wageningen
Organisation: Wageningen University
Promotor: prof.dr. R.G.F. Visser (Plant Breeding (Genetical Variation and Reproduction))
Promotor: Dr.ir. J.M. van Tuyl

 Longiflorum (L), Asiatic (A) and Oriental (O) lilies belong to section Leucolirion, Sinomartagon and Archelirion of genus Lilium respectively. The F1 interspecific hybrids i.e. Longiflorum × Asiatic (LA) and Oriental × Asiatic (OA) are promising in lily breeding for various agronomical traits. Genomic in situ hybridization (GISH) was applied to assess the ploidy level, intergenomic recombination, genome composition and the mode of origin of these F1 hybrids (LA and OA) backcrossed (BC) to Asiatic parents. Beside 2n gametes some F1 LA hybrids also produced n gametes resulting in diploid and allotriploids BC progenies. These diploid BC progenies have the potential of ‘analytic breeding’ in allopolyploids. Through GISH analysis recombination sites were determined in BC progenies of LA and OA hybrids and cytomolecular maps were constructed for three genomes of lily. Diversity Array Technology (DArT) was used to construct a molecular linkage map in a F1 LA population comprised of 382 markers located on 14 linkage groups.
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