Fluorescence microspectroscopy is a powerful tool to visualize and quantify protein interactions in biological systems and can be used to reveal structural features of isolated biomolecules.
Application of fluorescence microspectroscopic methods has been proved to be powerful tools to visualize and quantify protein interactions biological systems and to reveal structural features of isolated biomolecules.
The Biomolecular Imaging group utilizes fluorescence imaging and spectroscopy tools to visualize and quantify biological processes in vitro and in vivo. Biochemical and genetic approaches have identified molecular components facilitating proper biological functioning living systems. However, identification of the molecular composition of complexes is usually deduced from total cell extracts hence no spatial information is obtained. Biomolecular Imaging allows to reveal where, when and in which molecular composition biomolecules function in cellular systems. In our laboratory, we apply several imaging modules like confocal imaging, fluorescence lifetime imaging microscopy (FLIM) or fluorescence correlation spectroscopy (FCS) on microscopic objects. Current research focuses on the following topics:
- Generating optimal FRET pair for ARF dimerization
- Chemical screening of ARF modulators
- FRET analysis by time resolved acceptor fluorescence
- Encapsulation of proteins using polyelectrolytes