Laboratory of Biochemistry - Proteomics / Metabolomics

In order to understand biological mechanisms the interplay between proteins as well as the influence of proteins on the physiological condition of biological systems is of great importance. For this, advanced instrumental methodology is needed.

lab2.jpg

New technological developments for proteomics and metabolomics studies are created within our group and tested as well on selected biological problems. These biological systems are often human nutrition related subjects, as for instance the influence of food components on the mechanistic function of the estrogen receptor, on the influence of food components on the changes in human lipoprotein and plasma composition or on the differences in nutritional content of important crops (broccoli, melon, rice). However, also model plant systems are studied in order to understand effects of intracellular hormone on cell-elongation, or the effect of protein-protein interaction in cell-division.

Selected publications

Joosten, H.-J., Han, Y., Niu, W., Vervoort, J., Dunaway-Mariano, D., Schaap, P.J. Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker-based method (2008) Proteins: Structure, Function and Genetics, 70 (1), pp. 157-166.

Moco, S., Capanoglu, E., Tikunov, Y., Bino, R.J., Boyacioglu, D., Hall, R.D., Vervoort, J., Vos, R.C.H., Tissue specialization at the metabolite level is perceived during the development of tomato fruit. (2007) Journal of Experimental Botany, 58(15-16), pp. 4131-46.

Moco, S., Bino, R.J., De Vos, R.C.H., Vervoort, J., Metabolomics technologies and metabolite identification (2007) TrAC - Trends in Analytical Chemistry, 26 (9), pp. 855-866.

Tatsis, E.C., Boeren, S., Exarchou, V., Troganis, A.N., Vervoort, J., Gerothanassis, I.P. Identification of the major constituents of Hypericum perforatum by LC/SPE/NMR and/or LC/MS (2007) Phytochemistry, 68 (3), pp. 383-393.

Nenadis, N., Vervoort, J., Boeren, S., Tsimidou, M.Z. Syringa oblata Lindl var. alba as a source of oleuropetn and related compounds (2007) Journal of the Science of Food and Agriculture, 87 (1), pp. 160-166.

Zvinavashe, E., Murk, A.J., Vervoort, J., Soffers, A.E.M.F., Freidig, A., Rietjens, I.M.C.M. Quantum chemistry based quantitative structure-activity relationships for modeling the (sub)acute toxicity of substituted mononitrobenzenes in aquatic systems (2006) Environmental Toxicology and Chemistry, 25 (9), pp. 2313-2321.

Moco, S., Bino, R.J., Vorst, O., Verhoeven, H.A., De Groot, J., Van Beek, T.A., Vervoort, J., Ric De Vos, C.H. A liquid chromatography-mass spectrometry-based metabolome database for tomato (2006) Plant Physiology, 141 (4), pp. 1205-1218.

Exarchou, V., Fiamegos, Y.C., van Beek, T.A., Nanos, C., Vervoort, J. Hyphenated chromatographic techniques for the rapid screening and identification of antioxidants in methanolic extracts of pharmaceutically used plants (2006) Journal of Chromatography A, 1112 (1-2), pp. 293-302.

Karlova, R., Boeren, S., Russinova, E., Aker, J., Vervoort, J., De Vries, S. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex includes BRASSINOSTEROID-INSENSITIVE1 (2006) Plant Cell, 18 (3), pp. 626-638.

Fiamegos, Y.C., Nanos, C.G., Vervoort, J., Stalikas, C.D. Analytical procedure for the in-vial derivatization - Extraction of phenolic acids and flavonoids in methanolic and aqueous plant extracts followed by gas chromatography with mass-selective detection (2004) Journal of Chromatography A, 1041 (1-2), pp. 11-18.

Exarchou, V., Godejohann, M., Van Beek, T.A., Gerothanassis, I.P., Vervoort, J. LC-UV-Solid-Phase Extraction-NMR-MS Combined with a Cryogenic Flow Probe and Its Application to the Identification of Compounds Present in Greek Oregano (2003) Analytical Chemistry, 75 (22), pp. 6288-6294.