Genotype to Phenotype Modelling of Plant Adaptation

We are glad to announce a symposium on genotype to phenotype modelling in relation to adaptation and GxE. The symposium is organized by WUR-Biometris and the EU-project Whealbi, with a program subdivided in three sessions.

The first session will focus on the importance of collecting, generating and characterizing the genetic variation for traits contributing to adaptation.

The second session focuses on strategies for phenotypic characterization across environments.

Finally, we discuss statistical and crop growth modelling approaches to connect genotypes and phenotypes across environments.

Organised by Biometris (WUR), in collaboration with EU-project Whealbi

Thu 16 November 2017 08:30 to 17:00

Setup Campus WUR
Venue Orion, building number 103
Bronland 1
6708 WH Wageningen
+31 (0) 317 48 87 77
Price description Registration fee to be paid in cash at the registration desk
Price EUR30.00



Session I: Genetic variation

08:30-09:00      Registration and coffee (ground floor)


09:00-09:10      Opening (Fred van Eeuwijk, WUR)


09:10-09:40      Gilles Charmet (INRA, EU-Whealbi coord.)

The Whealbi (Wheat and Barley Legacy for Breeding Improvement) project, started beginning of 2014, stems from the strong conviction that to improve wheat and barley production in order to face severe global changes, we need to better exploit knowledge from basic science to develop new varieties and innovative cropping systems. It associates 18 partners in 9 countries. 

WHEALBI  combines genomics, genetics and agronomy to improve European wheat and barley production in competitive and sustainable cropping systems. It will generate original data from expressed genome sequences of 1000 wheat and barley genetic resources and provide models and tools to integrate these data in breeding programmes and crop management.

As first results, exome of 500 barley and 500 wheat accessions covering a wide range of diversity have been sequenced. For example in wheat, 680 000 SNP and structural variation have been found, of which # 600 000 in bread wheat. These data have been analysed to detect evolutionary pathways, signature of selection and quantitative trait loci. 

The results will be disseminated to a broad user community, highlighting the benefits and issues associated with the adoption of what is considered sustainable and environmentally friendly wheat and barley crop production in a European context.

09:40-10:10      Chris Schön (TUM)

Making maize genetic resources amenable to elite germplasm improvement

Genome-enabled strategies for harnessing untapped allelic variation of landraces are currently evolving. The success of such approaches depends on the sampling of source material. Most studies capitalize on maximizing diversity by sampling few individuals from many landraces covering a wide range of geographic regions. For the improvement of elite germplasm, an alternative approach might be more suitable, namely sampling many individuals from few pre-selected landraces. We show the impact of different sampling strategies on diversity parameters and LD based on high-density genotypic data of 35 European maize landraces. First results from genome wide association mapping and genomic prediction for quantitative traits segregating in doubled haploid lines derived from pre-selected landraces will be presented.

10:10-10:30      Cris Wijnen (WUR)


10:30-11:00      Coffee


Session II: Phenotypic characterization

11:00-11:45      Francois Tardieu (LEPSE)

Prediction of the genetic variability of yield in environmental scenarios: comparing statistical and dynamical approaches based on phenotyping

S Alvarez Prado, E Millet, S Lacube, B Parent, C Welcker, F van Eeuwijk, F Tardieu

We have developed two approaches to deal with the prediction of yield in a range of environmental scenarios  in 29 experiments in Europe. Both of them consist in predicting sensitivity to environmental condition rather than yield or traits themselves. In a previous study, allelic effects at QTLs of yield were modelled as functions of environmental scenarios.

The first approach consists in extending this study towards genomic prediction of the sensitivities of yield to light, temperature and water deficit during periods of time corresponding to phenological stages of each genotype. Sensitivities are predicted using genetic markers (QTLs or GP). Yields are simulated in each site by combining calculated sensitivities with environmental conditions averaged during considered phenological stages.

The second approach consists in (i) phenotyping the genetic variability of traits (e.g. stomatal conductance, radiation use efficiency, growth), (ii) calculating trait values from genetic markers and environmental conditions and (iii) simulating yields based on these traits by using a dynamic crop model. Noteworthy, steps (ii) and (iii) are performed simultaneously by the crop model with an hourly or daily time step.

The likely conclusion is that the first approach may allow better prediction in a range of environmental scenarios whose characteristics are close to those in experiments. The second approach is more flexible and, at least potentially, would allow prediction in new combinations of environmental conditions (e.g. climate change). 

11:45-12:30      Scott Chapman (CSIRO/UQ)


12:30-13:30      Lunch (de Spot, Orion ground floor)



Session III: Genotype to phenotype modelling

13:30-14.15       Charlie Messina (DuPont Pioneer)

Phenotypic prediction augmented through crop model-whole genome prediction: Application to ARGOS

Phenotypic prediction accuracy for yield and transgenic effects is limited in agricultural systems where Transgene-by-Genotype-by-Environment-by-Management (T×G×E×M) interactions are frequent. The fusion of crop growth models (CGMs) with whole genome prediction (WGP) methods was demonstrated to improve prediction accuracy. We demonstrate the CGM-WGP methodology for the prediction of the effects of ARGOS, a transgene that can improve drought tolerance in maize.

14.15-14.45       Ep Heuvelink (WUR)


14:45-15:15      Willem Kruijer (WUR)

Multi-environment genomic prediction: a penalized regression perspective

Willem Kruijer, Emilie Millet, François Tardieu, Claude Welcker and Fred van Eeuwijk 

Multi-environment trials are usually characterized by environmental covariates like maximum temperature. From a statistical perspective, these covariates are important for dissecting genotype by environment interaction, and genomic prediction in new environments. In view of the large numbers of covariates that are now often available, variable selection has become increasingly important. We show how this can be achieved with a variety of penalized regression approaches, using data from the DROPS network (Millet et al. 2016, Plant Phys.).

15:15-15:45      Coffee


15:45-16:30      Daniela Bustos-Korts (WUR)

Considerations from statistical and crop growth models to design strategies to predict plant adaptation across environments

Daniela Bustos-Korts, Marcos Malosetti, Martin Boer, Fred van Eeuwijk

Selection processes in plant breeding depend critically on the quality of phenotype predictions. The phenotype is classically predicted as a function of genotypic and environmental information. One aspect that determines the quality of phenotype prediction is the set of genotypes used to train the prediction model, especially when populations are structured. A second aspect that influences the accuracy of phenotype predictions is the choice of environments used to train the prediction model, which should capture the heterogeneity in the target population of environments. Prediction accuracy can also benefit from modelling the target trait and its underlying physiological components, when components have a high heritability and they are correlated to the target trait. Trait correlations are modified by environmental conditions and by the genetic architecture. Therefore, additional phenotyping might be advantageous in some environments but not in others. A combination of crop growth models and statistical models can help breeders to identify when phenotyping additional traits would pay off by a larger accuracy for the target trait. In this presentation, we will discuss and illustrate considerations that need to be taken into account when deciding which genotypes, traits and environments are used to collect phenotypic information to predict adaptation across multiple environments.

16:30-17:00      Fred van Eeuwijk (WUR) 


17.00-18:00      Drinks (de Spot, Orion ground floor)