PhD defence

Combining LC-MS/MS and genetic analysis to unravel plant hormone metabolism in Arabidopsis thaliana

PhD candidate mr. SS (Sang) Lee
Promotor Harro Bouwmeester
Co-promotor Dick dr. D (Dick) Vreugdenhil
Lidiya dr. LI (Lidiya) Sergeeva
Organisation Wageningen University, Laboratory of Plant Physiology
Date

Tue 21 January 2020 13:30 to 15:00

Venue Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary:

The aim of this thesis was to study natural variation of plant hormones in Arabidopsis. In Chapter 2, to obtain reliable quantitative data of levels of hormone using LC-MS, a set of methodological processes was evaluated to reduce interference of impurities in extracts and we proposed a series of steps and procedure to optimize the protocol for hormone analysis. In Chapter 3, we reported limited variations of hormone levels in roots of a set of ecotype, including correlations with mature root system architecture. Chapter 4 and 5 describe diverse quantitative trait loci (QTLs) that are responsible for levels of auxin, abscisic acids, cytokinins, salicylic acid and jasmonic acid in roots and leaves of chosen experimental populations. We evaluated a functional role of zeatin N-glucosyltransferase gene underlying a large-effect QTL in cytokinin metabolism and further analysis was followed such as metabolite-ratio QTLs between different groups of hormone.