The pMF1 vector (Figure 3) is equipped with the npt II gene for kanamycin selection of transgenic shoots.
Two additional pMF-versions, pMF2 and pMF3 are available in which the selection gene hpt (hygromycin resistance) and pat (phosphinothricin resistance), respectively, have been introduced into a unique Spe I-restriction site of pMF1. It is also possible to introduce reporter genes like gus or gfp at the Spe I-site for monitoring initial transformation efficiencies. All selection genes and reporter genes will ultimately be removed.
|RB, LB||Right and Left Border of T-DNA, respectively|
|rec-LBD||Fusion of Recombinase R-gene and Ligand Binding Domain|
|cod A-npt II||Fusion of cod A gene for negative selection on fluoro cytosine and npt II gene for positive selection on kanamycin|
|SpeI||Restriction site in which hpt II or pat genes have been inserted in pMF2 and pMF3, respectively|
|MCS||Multiple Cloning Site|