Microarray

With micro array technology we can measure gene expression levels of thousands of genes simultaneously.

Microarray technology consists of:

  • isolation of messenger RNA
  • conversion to complementary DNA
  • labelling with a fluorescence group
  • hybridisation by microarray

In this process, the DNA-molecules are labelled with a fluorescence group and bound to the corresponding gene on the array. We then read the signals of the microarray using a laser scanner. The intensity of the signals provides information about the level of expression on each gene in the array.

Applications

RIKILT uses micro array technology for:

  • research into the health effects of natural and bioactive substances (nutrigenomics)
  • effect studies of contaminants and GMOs (toxicogenomics)
  • tracing GMOs, antibiotic resistant genes and microorganisms in food

RIKILT has the expertise and infrastructure to hybridise and analyse Agilent arrays. We also produce in-house arrays. We use these in-house arrays for gene expression studies of tomatoes and potatoes and the detection of microorganisms.