How accurate are kinetic rate constants inferred from #smFRET trajectories?
Sonja Schmid led a multi-lab benchmark study in which 12 expert labs joined forces to answer this question in a unique community-driven effort over the past 3 years. The results of this first-ever international blind study on single-molecule FRET kinetics are now available to everyone as a preprint. In a true open science spirit, also all related data is made public, including benchmark datasets, all inferred values, scripts, and parameters.
Read the study here: https://doi.org/10.1101/2021.11.23.469671