Calcium is an ubiquitous intracellular messenger carrying out this function in many eukaryotic signalling pathways. In order to understand the mechanism of calcium homeostasis and calcium transients in cells and how their disturbances are associated with disease states, it is necessary to be able to measure calcium inside cells.
Calcium monitoring by the bioluminescence of calcium-regulated photoproteins is very attractive for this purpose because new approaches for application of photoproteins through their expression in cells or their compartments were recently developed.
Although significant progress has been achieved in understanding the bioluminescence mechanism, almost nothing is known about the first step of the reaction, i.e. the formation of a stabilized peroxygenated reaction intermediate from apo-photoprotein, coelenterazine, and molecular oxygen.
Thus, the main goal of our studies is to elucidate the mechanism of active photoprotein complex formation. In prospect, the knowledge of this mechanism will provide the fundamental basis for improving intracellular assays involving photoproteins.
Siberian Branch, Krasnoyarsk 660036, Russia
Sandwich PhD project
Link to Master Project: Mechanism of calcium-regulated photoproteins.pdf