What if we could generate fuels directly just from Carbon dioxide and sunlight? In fact, the tiny green single celled microalgae are capable of doing it. However, we need to engineer these solar powered microalgae to enhance the amount of fuels/valuable compounds that can be produced to make them a sustainable biofuel production platform. We are developing and using the advanced CRISPR/Cas systems to achieve this goal.
Nannochloropsis oceanica is a microalgae that has been noted for its increased capability to produce Triacylglycerols (TAG) and PUFA (Polyunsaturated Fatty Acids). TAGs are used for biofuel production and PUFA’s have exceptional health benefits. However, the present amounts of these compounds produced by our strain is not sufficient to commercialize the production of biofuels and health supplements.
Aim of the project
Metabolic engineering of the strain with a focus on our product of interest could develop this strain as a platform organism for sustainable production of biofuels and PUFAs. To perform metabolic engineering, efficient genome editing tools are required. We have developed a CRISPR-Cas based genome editing system for successful generation of knockout and knock in mutants of our strain.
We have presently two parallel research lines in this particular project.
- Further development of genome editing tools using CRISPR-Cas12a for multiplexed genome editing and gene silencing
- Metabolic engineering of the strain for enhancing the TAG and PUFA content of the strain
Interested in one of these projects? Please contact Mihris Naduthodi (email@example.com).
Progress of CRISPR-Cas based genome editing in Photosynthetic microbesBiotechnology Journal 13 (2018)9. - ISSN 1860-6768
CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1Biotechnology for Biofuels 12 (2019)1. - ISSN 1754-6834