Vaccine development against carp viruses. Integrating adaptive immunity

PhD defence

Vaccine development against carp viruses. Integrating adaptive immunity

PhD candidate ms. CWE (Carmen) Embregts
Promotor prof.dr.ir. GF (Geert) Wiegertjes
Co-promotor dr. M (Maria) Forlenza PhD
Organisation Wageningen University, Cell Biology and Immunology, Wageningen Institute of Animal Sciences (WIAS), Wageningen Institute of Animal Sciences (WIAS), Aquaculture and Fisheries
Date

Wed 23 May 2018 13:30 to 15:00

Venue Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary

Common carp, one of the most cultured fish species worldwide, is threatened by many diseases. In this research we developed and tested vaccines against two important carp viruses and besides the traditional injection-vaccination, we explored possibilities for oral (stress-free) vaccination. For one of the viruses we successfully developed a DNA vaccine that induced full protection after intra-muscular injection, but not after oral delivery. We found that intra-muscular injection of this vaccine induced a strong local response as well as a virus-specific B and T cell memory response. Furthermore, we characterized the immune response to non-successful vaccines and we discuss possible ways for improvement. By developing new assays we significantly increased our knowledge and understanding of B and T cells in common carp and we characterized their roles during infection and after vaccination.