My research group is interested in how developmental processes are controlled by transcription factors and chromatin modifications. We aim to unravel transcriptional networks underlying various processes such as flowering time regulation, floral organ development, fruit formation and embryogenesis. We apply various methods, such as ChIP-seq, RNA-seq, proteomics, microscopy, CRISPR/CAS9 technologies and in vitro assays, to build gene regulatory networks and study the role of genes and proteins involved in these developmental processes. We are using predominantly the model species Arabidopsis and tomato, but also aim to understand to what extent the networks and genes are conserved in other species, including crops.
A main question of our research is: How do Transcription factors work and what are their target genes? To answer this question we are studying the properties of transcription factors belonging to the MADS domain, AP2-like or TCP transcription factor families. Since these transcription factors form larger complexes we analyse the components of the complexes by immunoprecipitation followed by MS/MS (Smaczniak et al, 2012). Furthermore, we are interested in the target genes that they control. A standard technology in our lab is ChIP-seq to identify in vivo binding sites. In addition we use in vitro methods, such as EMSA and SELEX to understand the specificity of binding to certain DNA sequences. Our results show that the composition of the transcription factor complex determines in part the binding specificity to target DNA.
We aim to identify downstream target genes by ChIP-seq and RNA-seq approaches and decipher their role in various developmental processes, such as flowering, flower, fruit and embryo development by genetic and molecular studies. A more recent focus of the group are studies to understanding the role of promoter elements (CIS regulatory elements) and how they control transcription. For this purpose we make mutations in promoters using CRISPR/Cas9, aiming at modulating gene expression in vivo.
Group members and teams
Evolution transcription factor
Tomato Fruit development
Plant Reproduction, recent achievements and technologies
A Quantitative and Dynamic Model of the Arabidopsis Flowering Time Gene Regulatory NetworkPLoS ONE 10 (2015)2. - ISSN 1932-6203
The cell size distribution of tomato fruit can be changed by overexpression of CDKA1Plant Biotechnology Journal 13 (2015)2. - ISSN 1467-7644 - p. 259 - 268.
AIL and HDG proteins act antagonistically to control cell proliferationDevelopment 142 (2015). - ISSN 0950-1991 - p. 454 - 464.
Hoe planten zombies wordenWageningen
Aanwezigheid van een gen is nog lang geen garantie : Moleculaire en genetische kennis maakt sprongenOnder Glas 11 (2014)9. - p. 18 - 19.
Plasticity in Cell Division Patterns and Auxin Transport Dependency during in Vitro Embryogenesis in Brassica napusThe Plant Cell 26 (2014)6. - ISSN 1040-4651 - p. 2568 - 2581.
Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factorsin flower developmentGenome Biology 15 (2014)3. - ISSN 1474-7596
Transcriptional control of fleshy fruit development and ripeningJournal of Experimental Botany 65 (2014)16. - ISSN 0022-0957 - p. 4527 - 4541.
The (r)evolution of gene regulatory networks controlling Arabidopsis plant reproduction; a two decades historyJournal of Experimental Botany 65 (2014)17. - ISSN 0022-0957 - p. 4731 - 4745.