Characterization of Cladosporium fulvum secondary metabolome
Analysis of C. fulvum genome showed that it contains many genes predicted to be involved in secondary metabolite biosynthetic pathways. As fungal secondary metabolites are biologically active compounds, including host-specific toxins and mycotoxins. Those produced by C. fulvum may play an important role during tomato infection. The student will (i) isolate secondary metabolites from in vitro cultures and from infected tomato, (ii) perform bioassays with these extracts to identify biological activities, (iii) construct knock-out mutants of candidate secondary metabolism genes, and (iv) evaluate secondary metabolite production in these mutants.