Project
Methode ontwikkeling en surveys fycotoxinen t.b.v. WOT
Natural toxins in food (mycotoxins, marine biotoxins and phytotoxins) are important factors in the occurrence of disease symptoms. This project responds to the need for innovative (validated) chemical research methods that are more efficient and more effective than existing methods, and that can adequately replace the official bioassays using experimental animals for calculating marine biotoxin concentrations.
Goals
The project goals are to develop and validate a suitable analysis method for the enforcement of EU regulations, to publish this method and to obtain data on the occurrence and significance of several specific marine biotoxins. The project will also lead to greater understanding of the responsible algae, their cultivation and the production of the respective toxins and metabolites.
Method
The research will focus on the following topics:
- 'Conventional' method development, validation and efficiency improvement for, among other things, European regulations
- 'Innovative' method development and prototype evaluation
- Surveys and monitoring
- International collaboration
Project results
The research must ensure that the Netherlands is able to identify all relevant classes of marine biotoxins that can possibly be present in shellfish and crustaceans in Dutch coastal waters, but also in sea fish in general. To this end, a number of chemical methods will be developed for various classes of marine biotoxins, including paralytic shellfish toxins and palytoxins. In addition, a general chemical method will also be developed for screening different classes of marine biotoxins in various matrices. Apart from leading to the establishment of practical methods, the results will also be presented at Dutch and international symposia and within the EURL/NRL network. Furthermore, the project will increase knowledge of the production of marine biotoxins and metabolites from algae.
Publications
-
A generic LC-HRMS screening method for marine and freshwater phycotoxins in fish, shellfish, water, and supplements
Toxins 13 (2021)11. - ISSN 2072-6651 -
A strategy to replace the mouse bioassay for detecting and identifying lipophilic marine biotoxins by combining the neuro-2a bioassay and LC-MS/MS analysis
Marine Drugs 16 (2018)12. - ISSN 1660-3397 -
Broad screening of phycotoxins in tissue, water and food supplements with LC-hrMS
-
Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study
Journal of AOAC International 101 (2017)2. - ISSN 1060-3271 - p. 468 - 479. -
Development and validation of a maleimide-based enzyme-linked immunosorbent assay for the detection of tetrodotoxin in oysters and mussels
Talanta 176 (2018). - ISSN 0039-9140 - p. 659 - 666. -
First Report on the Occurrence of Tetrodotoxins in Bivalve Mollusks in The Netherlands
Toxins 10 (2018)11. - ISSN 2072-6651 -
Marine biotoxins and associated outbreaks following seafood consumption : Prevention and surveillance in the 21st century
Global Food Security 15 (2017). - ISSN 2211-9124 - p. 11 - 21. -
Marine neurotoxins: State of the art, bottlenecks, and perspectives for mode of action based methods of detection in seafood
Molecular Nutrition & Food Research 58 (2014)1. - ISSN 1613-4125 - p. 87 - 100. -
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography-mass spectrometry: inter-laboratory validation study
Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment 28 (2011)12. - ISSN 1944-0049 - p. 1745 - 1757. -
Quantitative determination of marine lipophilic toxins in shellfish using LC-MS/MS : international validation study - final report
Wageningen : RIKILT (Report / Rikilt 2011.008)
Natural toxins in food (mycotoxins, marine biotoxins and phytotoxins) are important factors in the occurrence of disease symptoms. This project responds to the need for innovative (validated) chemical research methods that are more efficient and more effective than existing methods, and that can adequately replace the official bioassays using experimental animals for calculating marine biotoxin concentrations.
Goals
The project goals are to develop and validate a suitable analysis method for the enforcement of EU regulations, to publish this method and to obtain data on the occurrence and significance of several specific marine biotoxins. The project will also lead to greater understanding of the responsible algae, their cultivation and the production of the respective toxins and metabolites.
Method
The research will focus on the following topics:
- 'Conventional' method development, validation and efficiency improvement for, among other things, European regulations
- 'Innovative' method development and prototype evaluation
- Surveys and monitoring
- International collaboration
Project results
The research must ensure that the Netherlands is able to identify all relevant classes of marine biotoxins that can possibly be present in shellfish and crustaceans in Dutch coastal waters, but also in sea fish in general. To this end, a number of chemical methods will be developed for various classes of marine biotoxins, including paralytic shellfish toxins and palytoxins. In addition, a general chemical method will also be developed for screening different classes of marine biotoxins in various matrices. Apart from leading to the establishment of practical methods, the results will also be presented at Dutch and international symposia and within the EURL/NRL network. Furthermore, the project will increase knowledge of the production of marine biotoxins and metabolites from algae.
Publications
-
A generic LC-HRMS screening method for marine and freshwater phycotoxins in fish, shellfish, water, and supplements
Toxins 13 (2021)11. - ISSN 2072-6651 -
A strategy to replace the mouse bioassay for detecting and identifying lipophilic marine biotoxins by combining the neuro-2a bioassay and LC-MS/MS analysis
Marine Drugs 16 (2018)12. - ISSN 1660-3397 -
Broad screening of phycotoxins in tissue, water and food supplements with LC-hrMS
-
Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study
Journal of AOAC International 101 (2017)2. - ISSN 1060-3271 - p. 468 - 479. -
Development and validation of a maleimide-based enzyme-linked immunosorbent assay for the detection of tetrodotoxin in oysters and mussels
Talanta 176 (2018). - ISSN 0039-9140 - p. 659 - 666. -
First Report on the Occurrence of Tetrodotoxins in Bivalve Mollusks in The Netherlands
Toxins 10 (2018)11. - ISSN 2072-6651 -
Marine biotoxins and associated outbreaks following seafood consumption : Prevention and surveillance in the 21st century
Global Food Security 15 (2017). - ISSN 2211-9124 - p. 11 - 21. -
Marine neurotoxins: State of the art, bottlenecks, and perspectives for mode of action based methods of detection in seafood
Molecular Nutrition & Food Research 58 (2014)1. - ISSN 1613-4125 - p. 87 - 100. -
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography-mass spectrometry: inter-laboratory validation study
Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment 28 (2011)12. - ISSN 1944-0049 - p. 1745 - 1757. -
Quantitative determination of marine lipophilic toxins in shellfish using LC-MS/MS : international validation study - final report
Wageningen : RIKILT (Report / Rikilt 2011.008)