In vitro embryogenesis is a basic plant breeding tool that is used to mass propagate lines for breeding and production purposes.
Although in vitro embryogenesis is widely used by plant breeders, there are large differences in the response of genetic backgrounds to in vitro regeneration conditions such that many species and genotypes within a species cannot be propagated in vitro. The inability to propagate plants in vitrolimits the speed at which new lines can be bred, but more importantly, limits the gene pool from which new varieties can be selected.
There are a large number of important crops that remain recalcitrant for propagation via in vitro embryogenesis. There is an urgent need to develop generic tools to obtain efficient in vitro embryogenesis in a germplasm-independent, non-transgenic manner.
Goal of this project is developing:
- differential proteomics technologies (SILAC) that can be used to identify proteins that regulate in vitro embryo production;
- tools to transiently deliver regulatory proteins that induce embryo production in culture.
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