TU-16022 Optimal diagnostics by using innovative detection methods

In recent years several innovative research in diagnostics using Next Generation Sequencing (NGS) has been performed. These technologies have a huge potential and could be used for much broader diagnostics. For this it is important that there is good coordination between the various parties and standardization of procedures and analysis methods. Cooperation as embodied in this PPP with key inspection services and research institution Wageningen Plant Research could play an important international role.

In order to deploy NGS for diagnostics further fundamental research is needed. Use of these new methods will, in combination with more traditional techniques, be more practical and should be used in conjunction with the entire chain (sampling, extraction, molecular methods and analysis). Which methods are most effective, and where in the chain? In addition, it is shown that NGS will give new insights in detection and diagnosis, for example in the field of phylogeny versus pathogenicity / function. The use of genomic information compared to the present use of a single gene or a few genes (so-called barcodes) for the diagnostics. This is called PanGenomics. The added value is to look instead of one piece of the puzzle, we look now at all the pieces of the puzzle simultaneously. This creates an overall picture, which allows to control things better or validate it more efficiently.