Collection development started in 2011 with the acquisition of the main Dutch varieties of cultivated lamb’s lettuce (Valerianella locusta). Subsequently, the collection was extended with cultivars and landraces originating from other European countries, in particular Germany and France, as far as the material was not yet properly conserved and distributed by other national genebanks.

In 2013 a start was made with the sampling of natural populations of V. locusta in the Netherlands, followed by the collecting of specimens of related species occurring in the Netherlands, including V. carinataV. dentata and V. rimosa. Currently (April 2020), 46 accessions are available for distribution, including seven represented by wild species. The collection may be extended with wild specimens in the future through collecting outside the Netherlands.