Protein Spectroscopy
Optical techniques are used to obtain information about the structure, interactions and dynamics of proteins.
Our equipment:
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Single-molecule Förster Resonance Energy Transfer (smFRET)
FRET is used to measure molecular scale distances (1-10 nm) between fluorescence tags in individual proteins / protein complexes / protein-DNA... -
Time correlated single photon counting
Cuvet based TCSPC is used for time-resolved fluorescence measurements. It can quantify the amount of FRET, or resolve the ultrafast events in... -
STREAK camera
Cuvet based time-resolved fluorescence spectroscopy with the streak camera, where changes in fluorescence spectra can be monitored with a time... -
Circular dichroism: steady-state and stopped-flow
Technique that is used to obtain structural information, for example about the arrangement of peptide bonds in secondary protein structure elements... -
Steady-state spectrofluorometry
Spectrophotometer that is used to record fluorescence excitation and emission spectra. -
Fluorescence anisotropy
Time-resolved fluorescence anisotropy is used to obtain information about molecular rotation and homo-FRET between proteins. -
Pulse-Amplitude-Modulation (PAM)
Our PAM (Pulse-Amplitude-Modulation) chlorophyll fluorometer consists of a basic PAM101 system by Walz.