Different approaches of combating bacterial canker in tomato: in pursuit of resistance

PhD defence

Different approaches of combating bacterial canker in tomato: in pursuit of resistance

PhD candidate Mas M (Mas) Mas Muniroh Binti Mohd Nadzir
Promotor prof.dr. RGF (Richard) Visser
Co-promotor dr.ir. AW (Sjaak) van Heusden
dr.ir. JM (Jan) van der Wolf
Organisation Wageningen University, Laboratory of Plant Breeding
Date

Fri 14 December 2018 11:00 to 12:30

Venue Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary

The quarantine pathogen, Clavibacter michiganensis subsp. michiganensis (Cmm), is one of the most important bacterial pathogens affecting tomatoes. The quarantine status and the devastating disease symptoms caused by Cmm are problematic for many parties. Even though resistance/tolerance breeding is one of the most important and successful ways to manage diseases, other paths can also be walked to manage disease symptoms. We developed a new and an efficient way to screen big tomato populations in vitro and used it to fine map the QTL on chromosome 7. Furthermore, susceptibility (S) gene, WAT1, reduced disease symptoms. Lastly, alternative approaches were applied to reduce Cmm disease symptoms by applying grafting and utilizing biocontrol agens. In conclusion, we developed a new protocol that could ease disease screening, fine mapped the QTL on chromosome 7, found a potential new tolerance against Cmm by using S-gene and tested two alternative approaches to minimize wilting symptom.