Seminar: Novel Developments in Fluorescence Microscopy

Fluorescence microscopy is a widely used method to visualize fluorescent (bio)molecules in in vitro and in vivo. Novel developments in fluorescence microscopy provide enhanced contrast, single-molecule specificity and sensitivity. During this seminar, fluorescence microscopy techniques that are available within Shared Research Facilities will be highlighted.

Organised by Shared Research Facilities, Laboratory of Biochemistry, Laboratory of Biophysics, Laboratory of Cell Biology, Wageningen Imaging Spectroscopy Hub (WISH)

Mon 20 November 2017 12:00 to 13:30

Venue Impulse, building number 115
Stippeneng 2
6708 WE Wageningen
+31 317-482828

We will discuss three imaging modules; spinning disk confocal microscopy, high resolution fluorescence microscopy and super-resolution microscopy. These imaging techniques, in particular when combined with single molecule sensitivity and optimized fluorescent probes, can provide dynamic information of (DNA-)protein interactions (in vitro or in vivo), signal transduction, molecular conformations, and self-assembly processes.

Please, join this free seminar if you are interested in learning about high resolution fluorescence microscopy and how this technology can be used in your own research. Coffee and tea will be served and please bring your own lunch. Please register beforehand.

12:00 hrs Short introduction on Shared Research Facilities
12:05 hrs Introduction to spinning disk confocal imaging - Marcel Janson
12:25 hrs Recent advances in confocal microscopy - Jan Willem Borst
12:45 hrs Fluorescence based super resolution microscopy and single particle tracking: an introduction - Johannes Hohlbein
13:05 hrs Discussion and Questions
13:30 hrs Closure

During the seminar, WUR-researchers will present different fluorescence microscopy applications ranging from signal transduction pathways in planta to monitoring CRISPR-Cas antiviral defence mechanisms in bacteria.