This platform is dedicated to the highly sensitive, quantitative, and targeted analysis of small molecules The standard set-up is used to analyse non-volatile compounds (mostly primary metabolites). The system is equipped with a sample preparation robot that is capable of automated online TMS derivatisation procedure especially suited for non-volatile compounds including amino acids, organic acids, mono- and disaccharides.
The analyses is done by a technique called multi reaction monitoring (MRM), where only specific target mass ions are selected by the first quadrupole and subsequently fragmented by the second quadrupole, to obtain a set of “daughter” mass ions. These “daughter” mass ions are highly specific for a particular analyte of interest. The third quadrupole is then used as a scanning device or mass filter. This allows a very sensitive detection of analytes even in complex sample matrices with a high dynamic range.
Next to the MRM analysis of target compounds the system can be run in full scan mode, simultaneously enabling the untargeted analysis of target and non-target compounds, including spectral matching of unknowns with mass spectral libraries (e.g., NIST 14).
Trace GC 1300
- PTV injector programmable with max. 15 °C/sec up to 450 °C
- Deans heartcut module
- Ready for one extra injection or detection system
- ExtractaBrite electron impact ion source cassette
- Mass range: 1.2-1100amu
- Scanning speed: up to 20,000amu/s
- Dynamic range: up to 7 orders of magnitude
- Simultaneous multimode acquisition (SIM, SCAN, SRM)
- Timed SRM function for efficient multi-compound analysis
TriPlus RSH injection robot
- Heated agitator for 6 samples
- Custom made sample tray for up to 220 samples
- Automatic syringe exchange system
- Automated online MeOX/TMS derivatisation procedure
- Targeted analysis of primary plant metabolites with relation to stress, resistance, crop quality, adaptation, etc.
- Untargeted metabolomics analysis with relation to e.g., plant-environment interactions, disease biomarkers, biosynthetic and signalling route elucidation, etc.