Obesity is a social health issue which is rapidly becoming a serious problem in the Western world. Obesity is one of the risk factors in metabolic syndrome.
The goal of this project is to study the effects of dietary fibre and protein hydrolysate on the gene expression profiles of cell lines in relation to satiety. This study is part of larger projects (IPOP Food, Fibre and Health and PIDON Obesitas) focusing on research on a range of related topics from food ingredients to human studies.
The results are as follows:
- Sets of differential genes which have been expressed (after incubation of various fibre and protein fractions with Hutu-80 and STC-1 cells).
- Analyses of relationships of these sets of genes with satiating effects in vivo. For instance, have any peptide hormones or their receptors been expressed which are linked to satiation?
- Comparisons of the results of these analyses with the results of human in vivo studies on satiation with fibres or proteins. In this way it will be possible to determine the value of the in vitro method for characterising fibre and protein fractions in relation to satiation.
In the final year of both the IPOP project Food, Fibre & Health and the PIDON project Obesitas, fibre fractions and protein hydrolysates will be incubated (with Hutu-80 and STC-1 cells).
Analyses of differential genes which have been expressed will be compared with the results of human in vivo studies carried out within both projects.
How do I look? Focusing attention on the outside body reduces responsiveness to internal signals in food intakeJournal of Experimental Social Psychology 56 (2015). - ISSN 0022-1031 - p. 207 - 213.
In vitro fermentation of 12 dietary fibres by faecal inoculum from pigs and humansFood Chemistry 133 (2012)3. - ISSN 0308-8146 - p. 889 - 897.
In vivo degradation of alginate in the presence and in the absence of resistant starchFood Chemistry 172 (2015). - ISSN 0308-8146 - p. 117 - 120.
Influence of a diet rich in resistant starch on the degradation of non-starch polysaccharides in the large intestine of pigsCarbohydrate Polymers 93 (2013)1. - ISSN 0144-8617 - p. 232 - 239.
Pectin is not pectin: A randomized trial on the effect of different physicochemical properties of dietary fiber on appetite and energy intakePhysiology and Behavior 128 (2014). - ISSN 0031-9384 - p. 212 - 219.
Satiety and energy intake after single and repeated exposure to gel-forming dietary fiber: post-ingestive effectsInternational Journal of Obesity 38 (2014). - ISSN 0307-0565 - p. 794 - 800.