The Bruker Avance III 400MHz NMR spectrometer

The Laboratory for Organic Chemistry has, within the context of the Wageningen NMR Center, a Bruker Avance III 400MHz NMR spectrometer at it’s disposal. The system includes a inverse broadband probe fitted with a Z-axis gradient and with automatic tuning and matching. The inner coil is optimized for 1H and the outer coil can be tuned from 31P to 97Mo (and others in between). So X-frequencies are ranging from 162 to 27 MHz. The whole system is controlled by Bruker’s Topspin software. A B-ACS 60 sample changer is present to be flexible in non-working hours.

Due to the presence of a gradient inverse probe 2D experiments like HSQC, HMQC, COSY and HMBC are easy to perform. Also 1H-experiments are easy due to the high sensitivity.