Zeiss Pascal CLSM

  • The PASCAL Zeiss Confocal Laser Scanning microscope is connected to the left port of an automated Axiovert 200M inverted microscope.
  • The system is equiped with a 25 mW Argon laser and a 1 mW HeNe laser providing four laserlines resp. 458-488-513 nm and 513 nm.
  • A transmission detector is implemented to obtain DIC images. This confocal has one PMT, which requires sequential imaging for multicolor detection.
  • Furthermore, the PASCAL is equiped with a Narishige micro-injection system to inject or manipulate living cells.