From 1-3 June 2010, an international Training Workshop was organized by the Centre for Genetic Resources of Wageningen University and Research Centre (CGN), in collaboration with and co-funded by the European Regional Focal Point for Animal Genetic Resources (ERFP) and the Food and Agricultural Organization of the United Nations (FAO). The aim of the Training Workshop was to transfer and exchange state of the art knowledge in the area of Gene Banking and to share experiences between European countries. Target group of this Training Workshop were professionals with strategic and/or operational responsibilities in Gene Banking in Europe. CGN welcomed more than 30 participants from 23 countries. The program consisted of a mixture of plenary lectures and discussions and hands-on experience with cryopreservation and analysis of genetic data. Lecturers were recruited from the scientific and Gene Banking community in Europe. The Training Workshop will contribute to strengthening of local, national and European Gene Banking and Cryopreservation strategies.