Perception of fungal effectors by plant receptors

Identification of a tomato co-receptor complex involved in Cf-9/Avr9 resistance signalling

Cf resistance proteins contain a transmembrane domain and a expected to recognize the cognate effectors peptide at the plasma membrane (PM). Interactors that could make a functional complex with Cf-9 likely belong to the family of RLKs. The studen t will (i) clone candidate genes in a binary vector for transiest expression, (ii) transiently co-express Cf-9-YFP and putative interactors in N. benthamiana, and (iii) check whether Cf-9-YFP remains in the ER or is relocalized to the PM byluoresence microscopy.