Event
Rhodobacter workshop I. - 2023
With this Rhodobacter workshop we would like to bring together for the first time the scientific community working with these intriguing microorganisms. It will be an informal workshop centered around eight topics (see below).
Topics
The topics of the Rhodobacter workshop are:
- Microbial diversity and ecology
- Genome editing
- Photosynthesis
- Gene regulation
- Metabolism
- Isoprenoid, Q10, carotenoid, hydrogen and PHB production
- Protein production
- Research tools
Rhodobacter species display a high metabolic versatility, being able to grow photo-, chemo-, auto- and heterotrophically. They are able to use a wide range of substrates, ranging from C1 compounds to fatty acids. They have been serving as model organisms for studying anoxygenic photosynthesis, metabolic regulation, chemotaxis and quorum sensing. They are natural producers of relevant bio-based compounds such as isoprenoids, PHB and hydrogen gas. The last years genome editing tools have become available that make it possible to develop Rhodobacter species as microbial cell factories.
The meeting will consist of keynote lectures, oral and poster presentations.
Registration
Registration will be open till 2 March 2023.
Cancellation is possible up to one month prior to the workshop (03-03-2023).
9:00 | Opening, Jules Beekwilder, Ruud Weusthuis |
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9:10 | Sponsor presentation |
Producing fragrance and flavour ingredients in Rhodobacter | |
Jules Beekwilder, Anouk Coenen, Estela Delgado, Frank Fluitman, Belén Infanzón, Elena Melillo, Wesley Roverts, Simone Schmitz, Robert Trokowski, Celina Vossen, Wouter Wisselink, Matthew Q. Styles | |
9:45 | Keynote. Modeling and mining the activities of Rhodobacter for society |
Timothy Donohue | |
10:20 | Coffee break |
Session 1 | Moderator: Elena Melillo (Isobionics) |
10:50 | Investigating and engineering carbon metabolism in Rhodobacterales |
L L. Schada von Borzyskowski, M. Ren, C. Iacometti, B. Pommerenke, N. Paczia, T. Glatter, T. J. Erb | |
11:15 | Pyoverdine-based interspecies interaction of P. putida with R. capsulatus |
Antonia Christine Zeisel, Andrea Jeanette Weiler, Luzie Kruse, Seung-Hyun Paik, Fabienne Knapp, Vera Svensson, Bastian Wollenhaupt, Johanna Wiechert, Dietrich Kohlheyer, Julia Frunzke, Karl-Erich Jaeger, Thomas Drepper | |
11:40 | Enhanced PHB production from Pseudohalocynthiibacter aestuariivivens |
Fortunato Palma Esposito, Vittoria Vecchiato, Carmine Buonocore, Pietro Tedesco, Brendon Noble, Pooja Basnett, Donatellade Pascale | |
12:05 | Lunch |
Session 2 | Moderator: Florence Schempp (BASF) |
13:05 | Keynote. Engineering terpenoid production in Rhodobacter capsulatus |
A. Loeschcke, J. Hage-Hülsmann, O. Klaus, F. Knapp, K. Troost, V. Svensson, A. Heck, K.E. Jaeger, T. Drepper | |
13:40 | Racing-red chassis: advancing Rhodobacter sphaeroides as cell factory for isoprenoid biosynthesis |
E. Orsi, J. Beekwilder, I. Mougiakos, P.L. Folch, V.T. Monje-López, B.M. Fernhout, A. Turcato, M.R. Ghiffary, W. Post, A. de Maria, B. Adiego-Perez, M. Dompè, S. Peek, D. van Gelder, A. van Houwelingen, J. Van Der Oost, G. Eggink, S.W.M. Kengen, R.A. Weusthuis | |
14:05 | Utilisation of plant biomass sugars by industrial microorganism Rhodobacter for production of high-value terpenes |
A. van Houwelingen, A. Vos, J, Beekwilder, G. Van Aken, D. Bosch, K. Cankar | |
14:30 | Elimination of competing pathways in terpene producing Cereibacter sphaeroides using appA and ppsR |
A.M. Vos, S. van der Laan, A. van Houwelingen, K. Cankar, F. Fluitman, J. Beekwilder, D. Bosch | |
14:55 | Tea break |
Session 3 | Moderator: Enrico Orsi (DTU) |
15:25 | Keynote: The photosynthetic apparatus of Rhodobacter sphaeroides |
C.N. Hunter | |
16:00 | Towards Photosystem III: Redesigning Reaction Centre Electron Transfer |
Adam G.M. Bowie, Andrew Hitchcock, David J. K. Swainsbury, George A. Sutherland & C. Neil Hunter | |
16:25 | Cost action: PurpleGain (https://www.cost.eu/actions/CA21146/) |
Daniel Melchor Puyol Santos | |
16:45 | End of day program |
19:00 | Workshop diner |
Session 4 | Moderator: Frank Fluitman (Isobionics) |
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9:00 | Keynote: Gene expression and regulation in Rhodobacter sphaeroides |
Matthew McIntosh, Jonas Kretz | |
9:35 | Identification of transcriptional regulators involved in isoprenoid biosynthesis in Rhodobacter sphaeroides |
Alice Di Girolamo, Janani Durairaj, Thamara Hesselink, Aurin Vos, Adèle van Houwelingen, Harro J. Bouwmeester, Aalt DJ. Van Dijk, Dick de Ridder, Jules Beekwilder | |
10:00 | Coffee break |
Session 5 | Moderator: Wouter Wisselink (Isobionics) |
10:30 | Temporal analysis of the R. sphaeroides transcriptome and proteome in response to oxygen depletion |
J. H. Salisbury, A. J. Taylor, J. Webb, P. J. Jackson, C. N. Hunter, A. Hitchcock | |
10:55 | Optogenetic switches for light-mediated control of microbial processes |
Fabienne Knapp, Nora Bitzenhofer, Oliver Klaus, Seung-Hyun Paik, Fabian Hogenkamp, Alina Burmeister, Alexander Grünberger, Dietrich Kohlheyer, Jörg Pietruszka, Karl-Erich Jaeger, Thomas Drepper | |
11:20 | Engineering nitrogenase enzymes in Rhodobacter capsulatus |
J.G. Rebelein | |
11:45 | Lunch Break |
Session 6 | Moderator: Oskar Zelder (BASF) |
12:45 | Keynote: Exploring Rhodobacter species and other purple bacteria for sustainable microbial protein applications |
Siegfried Vlaeminck | |
13:10 | Characterising electron transport to the Fe-nitrogenase in Rhodobacter capsulatus |
Holly Addison, Dr. Timo Glatter, Dr. Johannes Rebelein | |
13:35 | CO2 inhibits the Fe-only nitrogenase but not the Mo nitrogenase in vivo |
N.N. Oehlmann, J.G. Rebelein | |
14:00 | What causes the transition phase in Cereibacter sphaeroides heterotrophic growth? |
M. Kostanjšek, N. van der Straten, J. Beekwilder, S. Kengen, R. A. Weusthuis, Mark M.M. Bisschops | |
14:30 | End, Jules Beekwilder, Ruud Weusthuis |
Keynote speakers
Matthew McIntosh
Microbiology and Molecular Biology
Justus-Liebig-Universität, Giessen, Germany
Gene expression and regulation in Rhodobacter sphaeroides
C. Neil Hunter
Department of Molecular Biology and Biotechnology
University of Sheffield, UK
The photosynthetic apparatus of Rhodobacter sphaeroides
Keynote speakers
Matthew Styles
Isobionics BV
Geleen, The Netherlands
Producing fragrance and flavour ingredients in Rhodobacter
Anita Loeschcke
Molecular Enzyme Technology
Heinrich-Heine-Universität, Düsseldorf, Germany
Engineering terpenoid production in Rhodobacter capsulatus
Keynote speakers
Siegfried Vlaeminck
Department of Bioscience Engineering
Universiteit Antwerpen, Belgium
Exploring Rhodobacter species and other purple bacteria for sustainable microbial protein applications
Tim J. Donohue
Department of Bacteriology
University of Wisconsin, USA
Modeling and mining the activities of Rhodobacter for society