The assessment of sustainability at the level of the agri-food supply chain is complex.Achieving sustainability in agri-food chains requires coordinated interaction between chain actors. The aspect of coordination is rarely included in the assessment of sustainability and current assessment methodologies do not allow conclusions about the linkage between coordination and sustainability. This paper analyzes the state of the art in the assessment of agri-food supply chain sustainability based on a structured literature review. Following the structured review that includes category analysis and content analysis, we develop a comprehensive sustainability assessment framework for agri-food chains across multiple stages. The novelty of the framework is to incorporate the dimension of coordination across chain stages as a critical sustainability dimension. The study contributes to research on the assessment of agrifood supply chain sustainability by incorporating the role of coordination across chain stages and its relationship with economic, environmental, and social performance. This essential relationship between coordination and sustainability offers several areas of interest for future research. The study also contributes to practice by providing scholars, chain actors, and policymakers with directions for improving sustainable strategies.