Plant regeneration is essential for plant breeding, propagation and production processes and therefore directly affects the ability of the ‘green’ sector to develop new genotypes that are optimised for grower and consumer-based traits. Somatic embryogenesis (SE), is the process in which vegetative cells are induced to form embryos. These embryos can be germinated and grown into plantlets that are identical to (clones of) the mother plant. Many commercially interesting vegetable crops and genotypes are recalcitrant or poorly responsive for SE, hampering their cost effective entry onto the market. This proposal aims to use live imaging and cell-specfic molecular analyses to develop a detailed mechanistic model for somatic embryo induction, and to use this knowledge to obtain practical solutions for inducing efficient SE in a germplasm-independent manner.
A cautionary note on the use of split-YFP/BiFC in plant protein-protein interaction studies
International Journal of Molecular Sciences 15 (2014). - ISSN 1661-6596 - p. 9628 - 9643.
AIL and HDG proteins act antagonistically to control cell proliferation
Development 142 (2015). - ISSN 0950-1991 - p. 454 - 464.
AINTEGUMENTA-LIKE proteins: hubs in a plethora of networks
Trends in Plant Science 19 (2014)3. - ISSN 1360-1385 - p. 146 - 157.
Central Cell-Derived Peptides Regulate Early Embryo Patterning in Flowering Plants
Science 344 (2014)6180. - ISSN 0036-8075 - p. 168 - 172.
Pepper, Sweet (Capsicum annuum)
In: Agrobacterium Protocols: Volume 1 (third edition) / , Wang, K.. - New York : Springer Science+Business Media (Methods in Molecular Biology 1223) - ISBN 9781493916948 - p. 321 - 334.
Production of interspecific Campanula hybrids by ovule culture: exploring the effecto of ovule isolation time
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