Recently, the production of baby food has gained a lot of interest since manufacturers cannot fulfill the enormous demand for baby food from China. One of the ingredients in many baby milk powers are galacto-oligosaccharides (GOS).
GOS are comparable to carbohydrates present in human breast milk and are important in baby food because they play a role in the development of the immune system. This research has focused on a more efficient production of GOS. GOS are synthesized from lactose; a reaction that is catalyzed by an enzyme called β-galactosidase. During this study we mainly focused on highly concentrated systems and we have gained more insight in the enzyme mechanism. Besides, a new (continuous) process concept has been designed that uses less enzyme and results in a more efficient production of GOS. The use of concentrated systems and this continuous process should yield a more sustainable process that needs less raw materials, water, and energy.