As part of the PPS Kleinschalige bioraffinage project (WP1b), fresh Stevia material was used in the extraction of steviol glycosides using water acidified through conversion of sugar by microorganisms naturally present on the plant. Two successive harvests from the same plot were used. Previous experiments had resulted in high steviol glycoside extraction rates of 80 % to 90 % but the purity of the final extract was low (15 % to 20 % of steviol glycosides in the dry matter). The first batch of plants was used to test a clarification step by filtration on a small scale. A second batch of plants was used to perform clarification, purification using ultrafiltration, and concentration by nanofiltration on a larger scale.