Transport is one of the largest challenges faced by veal calves. The first transport occurs when calves are transported from the dairy farms to a collection center at 14-20 days of age. Then, with a second transport, calves are brought to the veal farms. During transport and at the collection center, calves are mixed and exposed to new environmental conditions and microorganisms. Consequently, transport is associated with a high level of stress and an increase in infectious disease incidence. As a consequence of stress, the metabolism, immunity and health of calves might be compromised. Previous studies explored effects of transport on hematological, metabolic and immunological variables of calves. However, these studies focused just on one single factor (e.g. transport duration or pre-transport nutrition) and not on a combination of multiple factors associated with transport. The current research aimed to investigate effects of a pre-transport diet, transport duration and type of vehicle on metabolic and immunological variables of young calves upon arrival at the veal farm.