A Kaleidoscopic Look at Vanillyl Alcohol Oxidase

Promotie

A Kaleidoscopic Look at Vanillyl Alcohol Oxidase

Promovendus ms. GA (Gudrun) Gygli MSc
Promotor prof.dr. WJH (Willem) van Berkel
Organisatie Wageningen University, Laboratory of Biochemistry
Datum

wo 18 april 2018 16:00 tot 17:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Short summary

Vanillyl alcohol oxidase can make vanillin, the major flavour component of vanilla. We have studied this enzyme in great detail to better understand how it works and how exactly it produces vanillin. We have identified two different paths that guide substrates to where the reaction takes place. Once there, some substrates form an adduct, preventing product formation. Based on our work, we predict that the size of the substrate determines if the adduct is formed, and that one amino acid in the active centre of the enzyme is crucial for this process.

Our work will help other researchers to improve this enzyme so that it can make vanillin more efficiently.