Root formation in Arabidopsis

Promotie

Adventitious root formation in Arabidopsis: underlying mechanisms and applications

Promovendus Mehdi M (Mehdi) Massoumi Bagherabadi
Copromotor dr. GJM (Geert-Jan) de Klerk
dr. FA (Frans) Krens
Organisatie Wageningen University, Laboratory of Plant Breeding
Datum

di 6 september 2016 16:00 tot 17:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592
Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.