Terrestrial laser scanning (TLS) is a valuable tool for assessing the woody structure of trees in an accurate, non-destructive and replicable way. In this thesis, I assessed this potential with tropical forest trees from Guyana, Indonesia, and Peru. I explored the use of 3D models from TLS point clouds to reconstruct the volume of trees and infer above-ground biomass and develop allometric models based on TLS-derived parameters. Moreover, I also addressed the reconstruction of the fi ne details of branching architecture of tropical trees and aims to provide a method to estimate ecological questions in tropical forests. These outcomes are a scientifi c contribution to the current development of new methods using TLS in tropical forests, especially the e fforts to measure forest carbon emissions and the provision of new insights into tropical forest ecology.