Bacillus cereus spore damage recovery and diversity in spore germination and carbohydrate utilisation

Promotie

Bacillus cereus spore damage recovery and diversity in spore germination and carbohydrate utilisation

Promovendus ms. AK (Alicja) Warda MSc
Promotor prof.dr. T (Tjakko) Abee
Copromotor dr.ir. MN (Masja) Nierop Groot
Organisatie Wageningen University, Food Microbiology Laboratory
Datum

vr 28 oktober 2016 13:30 tot 15:00

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary

Bacterial spores are robust survival vehicles that are highly resistant towards stress conditions including heat and other stresses commonly applied during food production and preservation. Heat treatments are conventionally applied in food processing to reduce the microbial load of food products, however, in compliance with consumer desire for products with higher sensory and nutritional values, the treatment intensity may become milder. Consequently, subpopulations of spores, including those of the toxin-producing food-borne human pathogen Bacillus cereus, may emerge that are sublethally damaged rather than inactivated conceivably causing quality and safety issues after repair and outgrowth. The research described in this thesis has added novel insights in B. cereus capacity to cope with spore damage and provided insights in the distribution and putative functionality of (sub)clusters of GRs and carbohydrate utilisation clusters. Knowledge on spore damage repair, germination and metabolism capacity adds to further understanding of B. cereus ecology and transmission capacity.