Rubber trees are mainly grown for their latex that can be processed into various rubber products. The seeds are currently not used and regarded as waste. Recently, however, there is a growing interest to use rubber seed oil as a feedstock for biodiesel production. The by-product of this process is protein-rich press cake. In Indonesian rural area where most rubber trees plantations are owned by smallholder farmers, protein from rubber seed press cake can be applied as animal feed. Rubber seed can provide for half of the required protein source for animal feed in Central Kalimantan, Indonesia. The protein also can be processed further to obtain high-value products. Enzyme was applied for selective production of amino acids from the protein. Amino acids are important supplement for food and feed industries, as well as potential feedstocks for biochemical production. This approach increases the feasibility of a biorefinery route from protein to these applications.