Phenotypic and genetic diversity of the species Lactobacillus rhamnosus

Promotie

Phenotypic and genetic diversity of the species Lactobacillus rhamnosus

Promovendus CD (Corina) Ceapa
Promotor prof.dr. M (Michiel) Kleerebezem
Copromotor dr. J (Jan) Knol
Copromotor dr. J Lambert
Organisatie Wageningen University, Leerstoelgroep Host Microbe Interactomics
Datum

di 12 januari 2016 13:30 tot 15:00

Locatie Aula, gebouwnummer 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

The thesis explores the diversity of Lactobacillus rhamnosus, a species from which strains are studied for their anti-inflammatory, anti-allergic, and diarrhea preventing effects. The work combines observations on the behaviour of the bacteria in a simplified laboratory setting (use of carbohydrates, immune modulation effects, anti-pathogenic effects) with genomic information obtained by sequencing, with the aim to pinpoint genes that could be relevant for bacterial survival and metabolic capacities. Phenotypic and genotypic profiling analyses congruently revealed that carbohydrate metabolism and transport is essential for this species’ adaptation to the environment. Genotype – phenotype correlation analysis enabled us to predict and then experimentally verify genes responsible for the utilization of L-Sorbose, L-Fucose α-D-Methyl Glycoside.