Fluorescence microscopy is a widely used method to visualize fluorescent (bio)molecules in in vitro and in vivo. Novel developments in fluorescence microscopy provide enhanced contrast, single-molecule specificity and sensitivity. During this seminar, fluorescence microscopy techniques that are available within Shared Research Facilities will be highlighted.
We will discuss three imaging modules; spinning disk confocal microscopy, high resolution fluorescence microscopy and super-resolution microscopy. These imaging techniques, in particular when combined with single molecule sensitivity and optimized fluorescent probes, can provide dynamic information of (DNA-)protein interactions (in vitro or in vivo), signal transduction, molecular conformations, and self-assembly processes.
Please, join this free seminar if you are interested in learning about high resolution fluorescence microscopy and how this technology can be used in your own research. Coffee and tea will be served and please bring your own lunch. Please register beforehand.
|12:00 hrs||Short introduction on Shared Research Facilities|
|12:05 hrs||Introduction to spinning disk confocal imaging - Marcel Janson|
|12:25 hrs||Recent advances in confocal microscopy - Jan Willem Borst|
|12:45 hrs||Fluorescence based super resolution microscopy and single particle tracking: an introduction - Johannes Hohlbein|
|13:05 hrs||Discussion and Questions|
During the seminar, WUR-researchers will present different fluorescence microscopy applications ranging from signal transduction pathways in planta to monitoring CRISPR-Cas antiviral defence mechanisms in bacteria.