The Seahorse XFe96 offers opportunities for analysing cellular metabolism in a wide range of sample types (from cultured cells to model organisms). It uses optical biosensors in cell culture microplate assays to measure in real-time different parameters, e.g., oxygen and pH, providing a detailed profile of the cellular metabolism. This makes the Seahorse suitable to study effects of food components and toxins on the metabolism.
Shared Research Facilities and Human and Animal Physiology are organizing this seminar. Scientists from supplier Agilent Technologies will introduce the Seahorse and show you its possibilities. Researchers from WUR and other universities will present their experiences with the Seahorse analyser. In the afternoon you can take part in a hands-on workshop in the labs of Human and Animal Physiology chair group where Agilent will demonstrate applications and train users on the Seahorse.
Cellular metabolism is the intricate network of pathways providing energy and building blocks. Disturbance of the metabolic network is an underlying mechanism of many diseases in humans, animals and plants. Understanding metabolic imbalances can lead to specific dietary interventions, targeted to prevent disease or to design novel therapies for human and animal diseases. Sample types can be plant or yeast cells, human and animal blood or tissue cells.
Seminar “Measuring mitochondria and metabolism with the Seahorse – New opportunities and applications”
9:30 Petra Caessens (WUR, Shared Research Facilities)
9:35 Daniel Gebhard (Agilent Technologies) "Introduction to the Seahorse technology”
10:00 Dennis Buurman (Agilent Technologies) "Bringing metabolomic pathways to life with real-time measures of cellular energy metabolism”
10:15 Jan van den Bossche (Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam) "Mitochondrial Dysfunction Prevents Repolarization of Inflammatory Macrophages”
11:15 Jaap Keijer (Human and Animal Physiology, Department Animal Sciences, Wageningen University) "Challenging metabolism by limiting oxygen”
12.00 End of seminar
12.30 – ca. 17.00 Seahorse demonstration and training workshop