The Journey of Prokaryotic Argonaute Proteins. From fundamentals to application

Promotie

The Journey of Prokaryotic Argonaute Proteins. From fundamentals to application

Promovendus ing. JW (Jorrit) Hegge MSc
Promotor prof.dr. J (John) van der Oost
Copromotor dr. RHJ (Raymond) Staals
Organisatie Wageningen University, Laboratory of Microbiology
Datum

vr 10 mei 2019 16:00 tot 17:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary

Argonaute proteins (Agos) form a diverse group of enzymes found in every domain of life. The Journey of prokaryotic Argonaute proteins (pAgos) described in this thesis begins with the characterization of a newly discovered pAgo from Clostridium butyricum (CbAgo). This Ago is the first example of a pAgo that efficiently cleaves single stranded DNA at 37°C, thereby paving the road towards the development of pAgo-based applications at moderate temperature, such as genome editing. The Journey ends with the successful utilization of previously characterized thermophilic pAgos from Thermus thermophilus (TtAgo) and Pyrococcus furiosus (PfAgo) in two novel pAgo-based molecular applications.