Tospovirus. Induction and Suppresion of RNA silencing

Promotie

Tospovirus – Inductie en Suppressie van RNA Silencing

Promovendus MH (Marcio) Oliveira Da Costa
Promotor prof.dr. JM (Just) Vlak
Copromotor dr.ir. RJM (Richard) Kormelink
Organisatie Wageningen University, Laboratorium voor Virologie
Datum

ma 20 juni 2016 11:00 tot 12:30

Locatie Aula, gebouwnummer 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592
Short summary
In plants, the RNA silencing mechanism plays an important role in antiviral defense. Tospoviruses are negative-strand RNA viruses of plants, and to counteract the antiviral RNA silencing response, they have evolved a silencing suppressor function in their NSs protein. The present research investigates the plant-tospovirus interaction with focus on antiviral RNA silencing, including the target sequences in the virus RNA and the role of NSs as part of tospovirus counterdefence strategy. Firstly, tospoviruses sequences are analysed as targets of RNA silencing and further analysis is performed on the sequence of the predicted hairpin from the intergenic region of the S RNA. Secondly, biochemical experiments are performed analyzing NSs affinity to dsRNA molecules. Lastly, NSs proteins from different tospoviruses are evaluated concerning their ability to suppress systemic silencing. The present thesis adds to our understanding of tospovirus-plant interaction.