Promotie

Needles in a haystack: Pursuing directed evolution of CRISPR-Cas protein for improved genome editing

Promovendus E (Evgenios) Bouzetos MSc
Externe promotor Prof. Dr John van der Oost
Copromotor dr. RHJ (Raymond) Staals
Organisatie Wageningen University, Laboratory of Microbiology
Datum

vr 4 oktober 2024 13:00 tot 14:30

Locatie Omnia, gebouwnummer 105
Hoge Steeg 2
6708 PH Wageningen
+31 (0)317 - 484 500
Zaal/kamer Auditorium

Samenvatting (Engelstalig)

CRISPR-Cas proteins have revolutionized genome editing. Nevertheless, optimization and tailoring of CRISPR-Cas systems are still necessary for optimal genome editing. In this thesis we pursued to develop new methods for the optimization of CRISPR-Cas proteins and applied already established methods aiming to improve a well-described Cas9 system. The described research finds significance in methods development and protein optimization and characterization.