Germination thresholds for uptake of black salsify in the collection are 80% for cultivated material and 60% for accessions of wild species. Salsify is a biannual cross-fertilizing crop. This means that only second year plants will produce flowers. The seed production is taking place under outside conditions in isolation plots.

To regenerate black salsify seeds are sown in April in sowing trays. After five weeks the plants are transplanted in the field. The first year most of them will not flower, so there is no need to isolate them. In November the roots are harvested and stored at 4 °C. In March of the second year, the roots are planted again in isolated plots at least 50 meters apart. Triticale is normally used as an isolation crop.On a minimum number of 20 plants seeds are produced. In June the first seeds can be harvested but seed production can take place until October. More details about the CGN regeneration procedures for black salsify can be found through the link in this page.