Combination of lab experiments, data analysis and seminar made training school successful

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Combination of lab experiments, data analysis and seminar made training school successful

Gepubliceerd op
18 september 2018

Wageningen University & Research, Animal Breeding and Genomics did organize a WIAS-COSTaction training school from 25-29 of June, 2018 about functional annotation.

The training school was a combination of wet-lab experiments (hands on ChIP-seq), data analysis (genome annotation) and a seminar with international guest speakers. The strength of this training school is the combination of wet-lab and data analysis and presentations of application of this field in a seminar.

ChIP-seq data

Within the training school we worked with a team of international trainers on data analysis and wet-lab ChIP-seq of cultured chicken fibroblast cells and porcine liver tissue in close collaboration with Diagenode. Nuclei from cultured cells or harvested from disaggregated liver tissue were prepared followed by DNA-protein crosslinking, chromatin shearing and immunoprecipitation. Quantitative PCR was used to check for final enrichment.

Functional animal genome annotation

The data analysis part was divided in building blocks (modules) including a module on working with command line software (Linux), data quality checks, analysis of whole genome sequence, expression and ChIP-seq data with a final integration of all datasets.

Worldwide interest

In total 17 participants from 8 countries and 3 continents did follow this training school. We were able to give 10 participants a Cost-action FAANG-Europe scholarships for travel and accommodation. There will be a new training school on this topic from 24 till 28 of June, 2019.

Lab experiments