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News letter no1 PalAdapt

Gepubliceerd op
9 september 2019

N°1 - August 2019

Coordinator: Eric Grenier, Igepp – eric.grenier@inra.fr – #PalAdapt

Site web PalAdapt

Preliminary results

All the new virulent populations of Globodera pallida found in Europe belong to the European gene pool and not to novel introductions into Europe

An Allele Specific PCR tool was shared by the INRA team with the project partners. This molecular tool allows the identification of G. pallida populations corresponding to the gene pool present in Europe or to other gene pools present in South America. Using this tool, JKI and WU were able to show that the virulent populations found in Germany and in the Netherlands since 2014 belong to the European gene pool.  Therefore, all the virulent populations of G. pallida found to date in Europe have adapted to potato resistance from already present populations.

Virulent field populations found in Germany tend to have bigger cysts than their avirulent counterpart as was already observed in selected experimental lineages

It was previously observed that virulent selected experimental G. pallida lineages present larger cysts than avirulent lineages on the susceptible cv. Désirée (Fournet et al., 2016). In order to have a first look on the fitness and life history traits of the new virulent field populations, JKI investigated the correlation between cyst size and virulence. The results clearly show that the German virulent field populations display significantly more larger cysts than the avirulent field populations. New experiments are planned to further investigate this correlation and the interest of cyst size monitoring as a proxy of nematode virulence.

Fournet et al. (2016). https://doi.org/10.1002/ece3.2079

 

 

Conferences and workshop

33th Symposium of the European Society of Nematologists (ESN 2018). 9-13thSeptember 2018, Ghent, Belgium

Grenier E., Kiewnick S., Goverse A., Helder H., Fournet S., Montarry J., Holterman M. & Smant G. PALADAPT, an EFSA funded project for monitoring and tackling genetic selection in the potato cyst nematode Globodera pallida

51th Annual meeting of the Organization of Nematologists of Tropical America (ONTA 2019). 21-25th July 2019, San Jose, Costa Rica

Grenier E., Kiewnick S., Smant G., Fournet S., Montarry J., Holterman M., Van de Elsen S., Helder J. & Goverse A. Monitoring and tackling genetic selection in the potato cyst nematode Globodera pallida

Workshop “in vitro and miniaturized cyst nematode resistance assays on potato: current use and future prospects” – 18-20th March 2019, Wageningen, The Netherlands

The workshop was organised by the WU Nematology team on the Wageningen campus. It was attended by 19 participants, including PalAdapt stakeholders affiliated to either potato breeding companies, NPPO, national safety organizations or reference laboratories for nematode resistance testing in potato.

The goals were (i) to learn and improve practical skills (methods and materials to use, do’s and don’ts), (ii) to know more about the parameters of in vitro and miniaturized resistance tests, (iii) to know more about PCN resistance phenotypes in potato (observation of the susceptible response and the rapid or delayed resistance response), (iv) to exchange expertise and knowhow about the different applications and methods. 

Several in vitro or miniaturized methods were presented of which each has its own advantage or limitations depending on the question addressed (i.e. identification of pathotypes, screening for resistance in breeding programs, studying of resistance mechanisms). Overall, the different methodologies presented already allow the addressing of a large panel of situations and questions, but a lack was identified regarding the use of a miniaturized assay based on potato seeds instead of tubers or cuttings.

The workshop has also highlighted that we are lacking an updated list of reference populations to include in our future assays and that in order to improve the confidence in the resistance scoring across Europe, instructions should be given regarding the rearing and control of the reference populations.

https://www.wur.nl/en/newsarticle/PALADAPT-workshop.htm