The Seahorse XFe96 analyzer has the capability of analyzing cellular metabolism of a wide range of sample types (from cultured cells to model organisms), making it a highly versatile research tool.
Cellular metabolism, the intricate network of pathways providing energy and building blocks, nowadays is the intense focus of both basic and applied research attention. Disturbance of the metabolic network is an underlying mechanism of many diseases in humans, animals and plants. Understanding metabolic imbalances can lead to specific dietary interventions, targeted to prevent disease or to design novel therapies for human and animal diseases.
- Targeting cellular metabolic pathways with dietary components in cultured cells
- Screening the effect of food components on mitochondrial function and toxicity
- The Seahorse can be exploited to perform as an alternative for metabolic analyses in small
- Analyzing metabolism of isolated blood cells or tissue cells after interventions
- Analyzing cellular metabolism of genetically altered C. elegans strains.
- Following metabolism during embryonic development (eg zebrafish)
- Analyzing fermentation in yeast cells for food processing technologies
- Assessment of plant cell metabolism
Proteomic and biochemical studies of lysine malonylation suggest its malonic aciduria-associated regulatory role in mitochondrial function and fatty acid oxidationMolecular and Cellular Proteomics 14 (2015)11. - ISSN 1535-9476 - p. 3056 - 3071.
SIRT1/PGC1a-dependent increase in oxidative phosphorylation supports chemotherapy resistance of colon cancerClinical Cancer Research 21 (2015)12. - ISSN 1078-0432 - p. 2870 - 2879.
A computational study of the Warburg effect identifies metabolic targets inhibiting cancer migration Yizhak K, Le Dévédec SE, Rogkoti VM, Baenke F, de Boer VCJ, Frezza C, Schulze A, van de Water B, Ruppin E. (2014) Molecular Systems Biology 10 (2014).
Inhibiting epigenetic enzymes to improve atherogenic macrophage functions Van den Bossche J, Neele AE, Hoeksema MA, de Heij F, Boshuizen MC, van der Velden S, de Boer VCJ, Reedquist KA, de Winther MP. (2014) Biochemical and Biophysical Research Communications 455 (2014).