Project

Target validation of putative histidine kinase inhibitors using a transcriptomic approach

Background

Recently identified putative histidine kinase inhibitors (Velikova et al. 2016, Sci Rep) loaded to nanoparticles for delivery of antibacterials to Gram negatives (Gr-) are bactericidal against Gr- such as  e.g. E. coli despite the lack of essential histidine kinases (HK) in this pathogen (Velikova et al. 2017, Nanomedicine). A possible reason for the observed bactericidal effect in Gr- despite the lack of essential HKs could be due to the inhibition of targets different than the HKs. To test this hypothesis we would like to employ RNAseq and study the effect of putative HKI on the gene expression in a relevant pathogen.

Objectives

To study the effect of a putative HKI on the gene expression in a relevant pathogen using RNAseq.

Methods

RNAseq

General microbiology techniques

Aseptic work

Contact

Nadya Velikova, nadya.velikova@wur.nl