Exploration of Microbial Reductive Dehalogenation in Marine Environments

Project

Exploration of Microbial Reductive Dehalogenation in Marine Environments

Reductive dehalogenation by anaerobic microorganisms is an important driver of the halogen and carbon cycling in marine environments.

Background

Halogenated organic compounds, organohalides, originated from natural and anthropogenic sources are widely distributed in the environment. Organohalide-respiring bacteria (OHRB) are a special group of microbes that can reductively dehalogenate organohalides. This reductive dehalogenation (RD) process is mediated by reductive dehalogenase enzymes (RDases), encoded by rdh genes. Up to now, most known OHRB and functionally characterised RDases have been obtained from terrestrial environments, whereas less information is available from marine environments despite being prolific sources of natural organohalides.

Aim

The main aims of this project are:

  1. Exploration of reductive dehalogenation potential from marine sediments;
  2. Isolation of novel marine OHRB; 3) physiological, biochemical and genomic characterization of the isolates;
  3. Physiological, biochemical and genomic characterization of the isolates;
  4. Application of genome-resolved metagenomics and meta-transcriptomic for Identification of potential OHRB in enrichment cultures that resist isolation.
Exploration of reductive dehalogenation in marine environments

Contact

Are you interested in working on this? Please contact me and we can arrange a thesis (Chen3.Zhang@wur.nl).